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Before agar, microbiologists had experimented with other foodstuffs as microbial media. They turned to substances rich in the starches, proteins, sugars, fats, and minerals that organisms need for growth, testing with broths, bread, potatoes, polenta, egg whites, coagulated blood serums, and gelatine. However, none worked particularly well: all were easily broken down by heat and microbial enzymes, and their surface, once colonized, became mushy and unsuitable for isolating microbes.
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關恆說，其中一部分選擇低調的原因，是因為把在新疆拍攝下來的影片發佈之後，遭遇到很多網絡上的攻擊與謾罵、個人資料被公開、在中國的家人也曾被警察問話。